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IID00030
 UniprotP18615
ProteinNegative elongation factor E
GeneNELFE
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
380
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 244-343 Monomer :
 Evidence NMR 2bz2 A Reference
       Region 2bz2 A 244-256 disorder
       Region 2bz2 A 257-335 order
       Region 2bz2 A 257-259 high_rmsd
       Region 2bz2 A 332-335 high_rmsd
       Region 2bz2 A 336-343 disorder
 Evidence NMR 2jx2 A Reference
       Region 2jx2 A 244-256 disorder
       Region 2jx2 A 257-343 order
       Region 2jx2 A 257-258 high_rmsd
       Region 2jx2 A 341-343 high_rmsd
 Evidence NMR 1x5p A Reference
       Region 1x5p A 254-337 order
       Region 1x5p A 254-259 high_rmsd
       Region 1x5p A 335-337 high_rmsd
Seq 360-380 Hetero trimer : IID00167Complex,IID00238Complex
 Evidence X-RAY 5oob Z Reference
       Region 5oob Z 360-360 disorder
       Region 5oob Z 361-372 order
       Region 5oob Z 373-378 disorder
       Region 5oob Z 379-380 order
 Evidence X-RAY 5oob E Reference
       Region 5oob E 360-360 disorder
       Region 5oob E 361-375 order
       Region 5oob E 376-377 disorder
       Region 5oob E 378-380 order
 Evidence X-RAY 5oob K Reference
       Region 5oob K 360-360 disorder
       Region 5oob K 361-374 order
       Region 5oob K 375-377 disorder
       Region 5oob K 378-380 order
SeqProS predicted 361-372 The unbound state of this region is predicted to be disordered by DICHOT and Foldindex Hetero trimer : IID00167Complex,IID00238Complex
       Region 5oob E 361-375 order
       Region 5oob K 361-374 order
       Region 5oob Z 361-372 order
Seqphosphorylation
    281-281 Phosphoserine
    353-353 Phosphoserine
    274-274 Phosphothreonine
    179-179 Phosphoserine
    113-113 Phosphoserine
    115-115 Phosphoserine
    131-131 Phosphoserine
    139-139 Phosphoserine
    165-165 Phosphoserine
    51-51 Phosphoserine
    181-181 Phosphoserine; by CDK9
    187-187 Phosphoserine; by CDK9
    185-185 Phosphoserine; by CDK9
    191-191 Phosphoserine; by CDK9
    249-249 Phosphoserine
    251-251 Phosphoserine
    272-272 Phosphothreonine
 
Prediction
NeProc
Disorder 1-7,36-255,338-357
Order 8-35,256-337,358-380
ProS 1-7,57-83,91-98,106-112,131-158,164-174,226-255,344-357
AlphaFold
Disorder 1-3,8-8,35-59,79-258,334-360,370-380
Order 4-7,9-34,60-78,259-333,361-369
Pfam Hmmer
PF00076 264-327 6.9e-13
SEG 14-43 ,147-163 ,184-243
Function
Function in SwissProt
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.