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IID00030
 UniprotP18615
ProteinNegative elongation factor E
GeneNELFE
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
380
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 244-343 Monomer :
 Evidence NMR 1x5p A Reference
       Region 1x5p A 254-337 order
       Region 1x5p A 254-259 high_rmsd
       Region 1x5p A 335-337 high_rmsd
 Evidence NMR 2bz2 A Reference
       Region 2bz2 A 244-256 disorder
       Region 2bz2 A 257-335 order
       Region 2bz2 A 257-259 high_rmsd
       Region 2bz2 A 332-335 high_rmsd
       Region 2bz2 A 336-343 disorder
 Evidence NMR 2jx2 A Reference
       Region 2jx2 A 244-256 disorder
       Region 2jx2 A 257-343 order
       Region 2jx2 A 257-258 high_rmsd
       Region 2jx2 A 341-343 high_rmsd
Seq 360-380 Hetero trimer : IID00167Complex,IID00238Complex
 Evidence X-RAY 5oob Z Reference
       Region 5oob Z 360-360 disorder
       Region 5oob Z 361-372 order
       Region 5oob Z 373-378 disorder
       Region 5oob Z 379-380 order
 Evidence X-RAY 5oob E Reference
       Region 5oob E 360-360 disorder
       Region 5oob E 361-375 order
       Region 5oob E 376-377 disorder
       Region 5oob E 378-380 order
 Evidence X-RAY 5oob K Reference
       Region 5oob K 360-360 disorder
       Region 5oob K 361-374 order
       Region 5oob K 375-377 disorder
       Region 5oob K 378-380 order
SeqProS predicted 361-372 The unbound state of this region is predicted to be disordered by DICHOT and Foldindex Hetero trimer : IID00167Complex,IID00238Complex
       Region 5oob E 361-375 order
       Region 5oob K 361-374 order
       Region 5oob Z 361-372 order
Seqphosphorylation
    281-281 Phosphoserine
    353-353 Phosphoserine
    274-274 Phosphothreonine
    179-179 Phosphoserine
    113-113 Phosphoserine
    115-115 Phosphoserine
    131-131 Phosphoserine
    139-139 Phosphoserine
    165-165 Phosphoserine
    51-51 Phosphoserine
    181-181 Phosphoserine; by CDK9
    187-187 Phosphoserine; by CDK9
    185-185 Phosphoserine; by CDK9
    191-191 Phosphoserine; by CDK9
    249-249 Phosphoserine
    251-251 Phosphoserine
    272-272 Phosphothreonine
 
Prediction
NeProc
Disorder 1-7,36-255,338-357
Order 8-35,256-337,358-380
ProS 1-7,57-83,91-98,106-112,131-158,164-174,226-255,344-357
AlphaFold
Disorder 1-3,8-8,35-59,79-258,334-360,370-380
Order 4-7,9-34,60-78,259-333,361-369
Pfam Hmmer
PF00076 264-327 6.9e-13
SEG 14-43 ,147-163 ,184-243
Function
Function in SwissProt
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:10199401). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed:11940650, PubMed:12612062). Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (PubMed:18303858, PubMed:27282391).
(Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.