IDEAL List

IDEAL

IID00035
UniprotP25054
ProteinAdenomatous polyposis coli protein
GeneAPC
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS Network xml rdf

Structure

Experiment

:order disorder conflict PDB cluster ProS Pfam Domain SEG

2843

order/disorder by at least rule

disorder by at least rule

order by at least rule

order/disorder by majority rule

Seq 2-55 Monomer :

Evidence X-RAY 1deb B Reference

Region 1deb B 2-2 disorder

Region 1deb B 3-55 order

Evidence X-RAY 1deb A Reference

Region 1deb A 2-55 order

Seq 126-250 Monomer :

Evidence X-RAY 1m5i A Reference

Region 1m5i A 126-129 disorder

Region 1m5i A 130-173 order

Region 1m5i A 174-178 disorder

Region 1m5i A 179-239 order

Region 1m5i A 240-250 disorder

Seq 303-739 Hetero dimer : IID00606Complex

Evidence X-RAY 3nmz B Reference

Region 3nmz B 303-325 disorder

Region 3nmz B 326-365 order

Region 3nmz B 366-371 disorder

Region 3nmz B 372-437 order

Region 3nmz B 438-439 disorder

Region 3nmz B 440-733 order

Region 3nmz B 734-739 disorder

Evidence X-RAY 3nmz A Reference

Region 3nmz A 303-325 disorder

Region 3nmz A 326-369 order

Region 3nmz A 370-372 disorder

Region 3nmz A 373-437 order

Region 3nmz A 438-439 disorder

Region 3nmz A 440-736 order

Region 3nmz A 737-739 disorder

Seq 396-732 Hetero dimer : IID00619Complex

Evidence X-RAY 3qhe C Reference

Region 3qhe C 396-401 disorder

Region 3qhe C 402-429 order

Region 3qhe C 430-436 disorder

Region 3qhe C 437-689 order

Region 3qhe C 690-693 disorder

Region 3qhe C 694-699 order

Region 3qhe C 700-700 disorder

Region 3qhe C 701-731 order

Region 3qhe C 732-732 disorder

Evidence X-RAY 3qhe A Reference

Region 3qhe A 396-400 disorder

Region 3qhe A 401-427 order

Region 3qhe A 428-435 disorder

Region 3qhe A 436-731 order

Region 3qhe A 732-732 disorder

Seq 396-775 Monomer :

Evidence X-RAY 3t7u B Reference

Region 3t7u B 407-424 order

Region 3t7u B 425-437 disorder

Region 3t7u B 438-488 order

Region 3t7u B 489-490 disorder

Region 3t7u B 491-624 order

Region 3t7u B 625-628 disorder

Region 3t7u B 629-734 order

Region 3t7u B 735-775 disorder

Evidence X-RAY 3t7u A Reference

Region 3t7u A 407-424 order

Region 3t7u A 425-437 disorder

Region 3t7u A 438-487 order

Region 3t7u A 488-491 disorder

Region 3t7u A 492-624 order

Region 3t7u A 625-628 disorder

Region 3t7u A 629-734 order

Region 3t7u A 735-775 disorder

Evidence X-RAY 3nmw B Reference

Region 3nmw B 407-428 order

Region 3nmw B 429-433 disorder

Region 3nmw B 434-738 order

Region 3nmw B 739-751 disorder

Evidence X-RAY 3nmw A Reference

Region 3nmw A 407-428 order

Region 3nmw A 429-433 disorder

Region 3nmw A 434-738 order

Region 3nmw A 739-751 disorder

Evidence X-RAY 5iza A Reference

Region 5iza A 407-743 order

Region 5iza A 744-751 disorder

Evidence X-RAY 5iz9 A Reference

Region 5iz9 A 407-430 order

Region 5iz9 A 431-435 disorder

Region 5iz9 A 436-738 order

Region 5iz9 A 739-751 disorder

Evidence X-RAY 5iz8 B Reference

Region 5iz8 B 407-701 order

Region 5iz8 B 702-704 disorder

Region 5iz8 B 705-724 order

Region 5iz8 B 725-751 disorder

Evidence X-RAY 5iz8 A Reference

Region 5iz8 A 407-743 order

Region 5iz8 A 744-751 disorder

Evidence X-RAY 5iz6 A Reference

Region 5iz6 A 407-427 order

Region 5iz6 A 428-435 disorder

Region 5iz6 A 436-741 order

Region 5iz6 A 742-751 disorder

Evidence X-RAY 5b6g A Reference

Region 5b6g A 407-427 order

Region 5b6g A 428-435 disorder

Region 5b6g A 436-741 order

Region 5b6g A 742-751 disorder

Evidence X-RAY 3au3 A Reference

Region 3au3 A 396-396 disorder

Region 3au3 A 397-689 order

Region 3au3 A 690-699 disorder

Region 3au3 A 700-707 order

Region 3au3 A 708-732 disorder

Seq 407-751 Hetero dimer : IID00606Complex

Evidence X-RAY 3nmx C Reference

Region 3nmx C 407-427 order

Region 3nmx C 428-439 disorder

Region 3nmx C 440-737 order

Region 3nmx C 738-751 disorder

Evidence X-RAY 3nmx B Reference

Region 3nmx B 407-426 order

Region 3nmx B 427-436 disorder

Region 3nmx B 437-737 order

Region 3nmx B 738-751 disorder

Evidence X-RAY 3nmx A Reference

Region 3nmx A 407-427 order

Region 3nmx A 428-435 disorder

Region 3nmx A 436-736 order

Region 3nmx A 737-751 disorder

Seq 407-751 Hetero dimer : Q5JTC6

Evidence X-RAY 4yjl F Reference

Region 4yjl F 407-738 order

Region 4yjl F 739-751 disorder

Evidence X-RAY 4yjl E Reference

Region 4yjl E 407-738 order

Region 4yjl E 739-751 disorder

Evidence X-RAY 4yjl D Reference

Region 4yjl D 407-738 order

Region 4yjl D 739-751 disorder

Evidence X-RAY 4yjl C Reference

Region 4yjl C 407-737 order

Region 4yjl C 738-751 disorder

Evidence X-RAY 4yjl B Reference

Region 4yjl B 407-737 order

Region 4yjl B 738-751 disorder

Evidence X-RAY 4yjl A Reference

Region 4yjl A 407-736 order

Region 4yjl A 737-751 disorder

Seq 407-751 Hetero dimer : Q5JTC6

Evidence X-RAY 4yk6 A Reference

Region 4yk6 A 407-741 order

Region 4yk6 A 742-751 disorder

Seq 407-751 Hetero dimer : Q5JTC6

Evidence X-RAY 4yje A Reference

Region 4yje A 407-738 order

Region 4yje A 739-751 disorder

Seqdisorder 1021-1035

Evidence CD Reference

Region 1021-1035 disorder

Seq 1021-1035 Hetero dimer : IID50011Complex

Evidence X-RAY 1jpp D Reference

Region 1jpp D 1021-1034 order

Region 1jpp D 1035-1035 disorder

Evidence X-RAY 1jpp C Reference

Region 1jpp C 1021-1031 order

Region 1jpp C 1032-1035 disorder

Seq 1362-1540 Hetero dimer : IID00039Complex

Evidence X-RAY 1th1 D Reference

Region 1th1 D 1362-1468 disorder

Region 1th1 D 1469-1496 order

Region 1th1 D 1497-1503 disorder

Region 1th1 D 1504-1529 order

Region 1th1 D 1530-1540 disorder

Evidence X-RAY 1th1 C Reference

Region 1th1 C 1362-1467 disorder

Region 1th1 C 1468-1496 order

Region 1th1 C 1497-1502 disorder

Region 1th1 C 1503-1529 order

Region 1th1 C 1530-1540 disorder

Seqdisorder 1362-1745

Evidence CD¸ NMR Reference

Region 1362-1745 disorder

Seq 1482-1528 Hetero dimer : IID50011Complex

Evidence X-RAY 1v18 B Reference

Region 1v18 B 1482-1484 disorder

Region 1v18 B 1485-1499 order

Region 1v18 B 1500-1503 disorder

Region 1v18 B 1504-1528 order

Seq 1484-1528 Hetero trimer : IID00039Complex

Evidence X-RAY 1t08 C Reference

Region 1t08 C 1484-1498 order

Region 1t08 C 1499-1528 disorder

Seq 1578-1596 Hetero dimer : IID00333Complex

Evidence NMR 2rqu B Reference

Region 2rqu B 1578-1596 order

Region 2rqu B 1594-1596 high_rmsd

Seq 2034-2059 Hetero dimer : IID00007Complex

Evidence X-RAY 1emu B Reference

Region 1emu B 2034-2049 order

Region 1emu B 2050-2059 disorder

Seq 2833-2843 Hetero dimer : IID00513Complex

Evidence X-RAY 3rl7 L Reference

Region 3rl7 L 2833-2838 disorder

Region 3rl7 L 2839-2842 order

Region 3rl7 L 2843-2843 disorder

Evidence X-RAY 3rl7 K Reference

Region 3rl7 K 2833-2838 disorder

Region 3rl7 K 2839-2843 order

Evidence X-RAY 3rl7 I Reference

Region 3rl7 I 2833-2837 disorder

Region 3rl7 I 2838-2842 order

Region 3rl7 I 2843-2843 disorder

Evidence X-RAY 3rl7 J Reference

Region 3rl7 J 2833-2837 disorder

Region 3rl7 J 2838-2843 order

Evidence X-RAY 3rl7 H Reference

Region 3rl7 H 2833-2837 disorder

Region 3rl7 H 2838-2842 order

Region 3rl7 H 2843-2843 disorder

Evidence X-RAY 3rl7 G Reference

Region 3rl7 G 2833-2836 disorder

Region 3rl7 G 2837-2843 order

Seq 2833-2843 Hetero hexamer : IID00513Complex

Evidence X-RAY 3rl8 F Reference

Region 3rl8 F 2833-2837 disorder

Region 3rl8 F 2838-2843 order

Seq 2833-2843 Hetero dimer : IID00513Complex

Evidence X-RAY 4g69 B Reference

Region 4g69 B 2833-2837 disorder

Region 4g69 B 2838-2843 order

SeqProS verified 1021-1034 Hetero dimer : IID50011Complex

Region 1jpp D 1021-1034 order

Region 1jpp C 1021-1031 order

Region 1021-1035 disorder

SeqProS verified 1468-1496,1503-1529

This region is ordered in the phosphorylated form, but disordered in the unphosphorylated form. Hetero dimer : IID00039Complex

Region 1th1 C 1468-1496 order

Region 1th1 D 1469-1496 order

Region 1th1 C 1503-1529 order

Region 1th1 D 1504-1529 order

Region 1362-1745 disorder

SeqProS verified 1484-1498 Hetero trimer : IID00039Complex

Region 1t08 C 1484-1498 order

Region 1362-1745 disorder

SeqProS verified 1485-1499,1504-1528

This region is ordered in the phosphorylated form, but disordered in the unphosphorylated form. Hetero dimer : IID50011Complex

Region 1v18 B 1485-1499 order

Region 1v18 B 1504-1528 order

Region 1362-1745 disorder

SeqProS verified 1578-1596 Hetero dimer : IID00333Complex

Region 2rqu B 1578-1596 order

Region 1362-1745 disorder

SeqProS possible 2034-2049

APC has three Axin binding repeats, SAMP1, SAMP2, and SAMP3. Because SAMP1 (1578-1596) is shown to be a verified ProS, this region, SAMP3, is possibly also ProS. Hetero dimer : IID00007Complex

Region 1emu B 2034-2049 order

SeqProS possible 2837-2843

This region is described to be disordered in free state (PubMed=15958564, 21859464). Hetero dimer : IID00513Complex

Region 4g69 B 2838-2843 order

SeqProS possible 2837-2843

This region is described to be disordered in free state (PubMed=15958564, 21859464). Hetero dimer : IID00513Complex

Region 3rl7 G 2837-2843 order

Region 3rl7 H 2838-2842 order

Region 3rl7 I 2838-2842 order

Region 3rl7 J 2838-2843 order

Region 3rl7 K 2839-2843 order

Region 3rl7 L 2839-2842 order

SeqProS possible 2837-2843

This region is described to be disordered in free state (PubMed=15958564, 21859464). Hetero hexamer : IID00513Complex

Region 3rl8 F 2838-2843 order

Seqphosphorylation

2710-2710 Phosphoserine

2724-2724 Phosphoserine

2789-2789 Phosphoserine

2674-2674 Phosphoserine

2679-2679 Phosphothreonine

2569-2569 Phosphoserine

2671-2671 Phosphoserine

2535-2535 Phosphoserine

2132-2132 Phosphoserine

2151-2151 Phosphothreonine

2260-2260 Phosphoserine

2270-2270 Phosphoserine

2283-2283 Phosphoserine

2473-2473 Phosphoserine

2125-2125 Phosphoserine

2129-2129 Phosphoserine

2130-2130 Phosphoserine

2093-2093 Phosphoserine

1973-1973 Phosphoserine

2088-2088 Phosphoserine

1971-1971 Phosphoserine

1861-1861 Phosphoserine

1864-1864 Phosphoserine

1863-1863 Phosphoserine

1774-1774 Phosphoserine

1567-1567 Phosphoserine

1395-1395 Phosphoserine

1371-1371 Phosphoserine

1438-1438 Phosphothreonine

1392-1392 Phosphoserine

1385-1385 Phosphoserine

1360-1360 Phosphoserine

1042-1042 Phosphoserine

1038-1038 Phosphoserine

987-987 Phosphoserine

908-908 Phosphoserine

780-780 Phosphoserine

744-744 Phosphoserine

748-748 Phosphoserine

111-111 Phosphoserine

107-107 Phosphoserine

Seqacetylation

2-2 N-acetylalanine

Prediction

NeProc

Disorder 1-1,69-126,169-185,249-306,366-373,428-437,729-1542,1571-2843

Order 2-68,127-168,186-248,307-365,374-427,438-728,1543-1570

ProS 69-89,122-126,169-185,263-269,297-306,433-437,729-747,772-779,790-814,824-829,885-893,909-916,930-937,972-986,1002-1006,1014-1019,1132-1136,1144-1149,1159-1165,1173-1188,1261-1276,1349-1352,1374-1387,1473-1480,1488-1493,1498-1501,1613-1617,1639-1652,1660-1663,1722-1736,1785-1791,2782-2786,2837-2843

AlphaFold

Disorder 1-6,8-8,51-130,174-178,237-325,363-373,427-443,627-627,737-752,760-2843

Order 7-7,9-50,131-173,179-236,326-362,374-426,444-626,628-736,753-759

Pfam Hmmer

PF00514 512-553 1.5e-05

PF00514 556-597 0.00035

PF00514 649-689 2.2e-10

PF00514 691-731 1.1e-05

PF05972 1020-1035 3.6e-06

PF05972 1136-1151 4.1e-05

PF05972 1155-1170 8.6e-06

PF05972 1172-1187 3.4e-06

PF05923 1256-1281 6.3e-11

PF05923 1369-1394 9.4e-08

PF05923 1485-1510 7.4e-07

PF05924 1568-1589 5e-10

PF05923 1636-1661 1.5e-08

PF05924 1718-1738 5.5e-10

PF05923 1840-1866 3.4e-07

PF05923 1948-1973 1.4e-07

PF05923 2006-2031 2.8e-08

PF05924 2033-2053 2.4e-09

PF05937 2670-2843 2.7e-84

SEG 94-111 ,134-156 ,213-224 ,290-299 ,833-846 ,959-971 ,1143-1158 ,1189-1224 ,1275-1282 ,1334-1346 ,1355-1366 ,1432-1450 ,1469-1481 ,1501-1511 ,1557-1581 ,1753-1759 ,1860-1875 ,1967-1973 ,1978-1993 ,2025-2039 ,2113-2146 ,2179-2193 ,2239-2247 ,2348-2369 ,2456-2477 ,2569-2590 ,2763-2774

Function

Function in SwissProt

Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

Biological Process

Diagram with PDB data
APC/CTNNB1	Beta-catenin in complex with a phosphorylated APC 20aa repeat fragment

See also
Diagram with PDB data
AXIN1/GSK3B	Crystal structure of GSK-3/Axin complex bound to phosphorylated N-terminal auto-inhibitory pS9 peptide