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IID00044
UniprotP39748
ProteinFlap endonuclease 1
GeneFEN1
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
380
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 1-336 Monomer :
 Evidence X-RAY 5um9 A Reference
       Region 5um9 A 2-336 order
 Evidence X-RAY 3q8m B Reference
       Region 3q8m B 2-335 order
       Region 3q8m B 336-336 disorder
 Evidence X-RAY 3q8m A Reference
       Region 3q8m A 2-336 order
 Evidence X-RAY 3q8l A Reference
       Region 3q8l A 2-336 order
 Evidence X-RAY 3q8k A Reference
       Region 3q8k A 2-336 order
 Evidence X-RAY 5kse A Reference
       Region 5kse A 2-336 order
 Evidence X-RAY 5k97 A Reference
       Region 5k97 A 2-336 order
 Evidence X-RAY 5fv7 B Reference
       Region 5fv7 B 1-1 disorder
       Region 5fv7 B 2-43 order
       Region 5fv7 B 44-59 disorder
       Region 5fv7 B 60-93 order
       Region 5fv7 B 94-130 disorder
       Region 5fv7 B 131-194 order
       Region 5fv7 B 195-201 disorder
       Region 5fv7 B 202-335 order
       Region 5fv7 B 336-336 disorder
 Evidence X-RAY 5fv7 A Reference
       Region 5fv7 A 1-1 disorder
       Region 5fv7 A 2-44 order
       Region 5fv7 A 45-59 disorder
       Region 5fv7 A 60-96 order
       Region 5fv7 A 97-130 disorder
       Region 5fv7 A 131-195 order
       Region 5fv7 A 196-200 disorder
       Region 5fv7 A 201-336 order
Seq 2-380 Hetero hexamer : IID00022Complex
 Evidence X-RAY 1ul1 Z Reference
       Region 1ul1 Z 2-113 order
       Region 1ul1 Z 114-119 disorder
       Region 1ul1 Z 120-356 order
       Region 1ul1 Z 357-380 disorder
 Evidence X-RAY 1ul1 Y Reference
       Region 1ul1 Y 2-44 order
       Region 1ul1 Y 45-59 disorder
       Region 1ul1 Y 60-102 order
       Region 1ul1 Y 103-133 disorder
       Region 1ul1 Y 134-359 order
       Region 1ul1 Y 360-380 disorder
 Evidence X-RAY 1ul1 X Reference
       Region 1ul1 X 2-46 order
       Region 1ul1 X 47-59 disorder
       Region 1ul1 X 60-99 order
       Region 1ul1 X 100-128 disorder
       Region 1ul1 X 129-198 order
       Region 1ul1 X 199-199 disorder
       Region 1ul1 X 200-357 order
       Region 1ul1 X 358-380 disorder
Seq 331-350 Hetero hexamer : IID00022Complex
 Evidence X-RAY 1u7b B Reference
       Region 1u7b B 331-335 disorder
       Region 1u7b B 336-348 order
       Region 1u7b B 349-350 disorder
Seq 335-350 Hetero tetramer : IID00022Complex
 Evidence X-RAY 5e0v D Reference
       Region 5e0v D 335-347 order
       Region 5e0v D 348-350 disorder
 Evidence X-RAY 5e0v C Reference
       Region 5e0v C 335-348 order
       Region 5e0v C 349-350 disorder
Seq 352-370 Hetero dimer : IID50009Complex
 Evidence X-RAY 3uvu B Reference
       Region 3uvu B 352-370 order
SeqProS verified 45-59,103-133 This region interacts with DNA :
       Region 3q8l A 2-336 order
       Region 3q8k A 2-336 order
       Region 3q8m A 2-336 order
       Region 3q8m B 2-335 order
       Region 5kse A 2-336 order
       Region 5k97 A 2-336 order
       Region 5um9 A 2-336 order
       Region 1ul1 Y 45-59 disorder
       Region 1ul1 Y 103-133 disorder
SeqProS possible 335-347 PIP box motif. Hetero hexamer : IID00022Complex
       Region 1u7b B 336-348 order
SeqProS possible 335-347 PIP box motif. Hetero tetramer : IID00022Complex
       Region 5e0v C 335-348 order
       Region 5e0v D 335-347 order
SeqProS verified 357-370 Hetero dimer : IID50009Complex
       Region 3uvu B 352-370 order
       Region 1ul1 Z 357-380 disorder
Seqphosphorylation
    364-364 Phosphothreonine
    336-336 Phosphothreonine
    335-335 Phosphoserine
    255-255 Phosphoserine
    293-293 Phosphoserine
    197-197 Phosphoserine
    187-187 Phosphoserine; by CDK2
Seqacetylation
    80-80 N6-acetyllysine
    375-375 N6-acetyllysine
    380-380 N6-acetyllysine
    377-377 N6-acetyllysine
    354-354 N6-acetyllysine
 
Prediction
NeProc
Disorder 334-380
Order 1-196,202-333
ProS 334-346
AlphaFold
Disorder 1-1,334-380
Order 2-333
Pfam Hmmer
PF00752 1-107 1.5e-64
PF00867 146-237 4.1e-53
PF01367 218-308 0.00027
SEG 105-124 ,361-380
Function
Function in SwissProt
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.