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IID00065
UniprotQ01167
ProteinForkhead box protein K2
GeneFOXK2
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
xml
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
660
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 256-353 Monomer :
 Evidence NMR 1jxs A Reference
       Region 1jxs A 256-353 order
       Region 1jxs A 256-256 high_rmsd
       Region 1jxs A 322-328 high_rmsd
       Region 1jxs A 351-353 high_rmsd
Seq 256-353 Homo dimer :
 Evidence X-RAY 2c6y B Reference
       Region 2c6y B 256-350 order
       Region 2c6y B 351-353 disorder
 Evidence X-RAY 2c6y A Reference
       Region 2c6y A 256-353 order
Seqphosphorylation
    30-30 Phosphoserine
    239-239 Phosphoserine
    373-373 Phosphoserine; by CDK1 and CDK2
    398-398 Phosphoserine
    424-424 Phosphoserine
    428-428 Phosphoserine; by CDK1 and CDK2
    599-599 Phosphoserine
Seqacetylation
    2-2 N-acetylalanine
 
Prediction
NeProc
Disorder 1-31,96-100,163-255,348-660
Order 32-95,106-162,256-347
ProS 1-9,163-171,180-197,208-210,348-352,365-371,467-472,482-486,556-560,571-581,654-660
AlphaFold
Disorder 1-34,62-62,87-109,158-255,352-660
Order 35-61,63-86,110-157,256-351
Pfam Hmmer
PF00498 54-146 9.7e-07
PF00250 258-353 1.4e-66
SEG 2-34 ,85-101 ,449-476 ,500-513 ,545-564
Function
Function in SwissProt
Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis and autophagy (By similarity). Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context (PubMed:22083952, PubMed:25451922). Together with FOXK1, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen (By similarity). Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4 (By similarity). Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate (By similarity). Together with FOXK1, acts as a negative regulator of autophagy in skeletal muscle: in response to starvation, enters the nucleus, binds the promoters of autophagy genes and represses their expression, preventing proteolysis of skeletal muscle proteins (By similarity). In addition to the 5'-GTAAACA-3' DNA motif, also binds the 5'-TGANTCA-3' palindromic DNA motif, and co-associates with JUN/AP-1 to activate transcription (PubMed:22083952). Also able to bind to a minimal DNA heteroduplex containing a G/T-mismatch with 5'-TRT[G/T]NB-3' sequence (PubMed:20097901). Binds to NFAT-like motifs (purine-rich) in the IL2 promoter (PubMed:1339390). Positively regulates WNT/beta-catenin signaling by translocating DVL proteins into the nucleus (PubMed:25805136). Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements (PubMed:1909027).