IDEAL List

IDEAL

IID00079
UniprotQ14653
ProteinInterferon regulatory factor 3
GeneIRF3
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
Network xml rdf

Structure

Experiment

:order disorder conflict PDB cluster ProS Pfam Domain SEG

427

order/disorder by at least rule

disorder by at least rule

order by at least rule

order/disorder by majority rule

Seq 1-112 Hetero tetramer : IID00441Complex

Evidence X-RAY 1t2k B Reference

Region 1t2k B 1-3 disorder

Region 1t2k B 4-111 order

Region 1t2k B 112-112 disorder

Evidence X-RAY 1t2k A Reference

Region 1t2k A 1-2 disorder

Region 1t2k A 3-112 order

Seq 1-113 Monomer :

Evidence X-RAY 3qu6 C Reference

Region 3qu6 C 1-4 disorder

Region 3qu6 C 5-39 order

Region 3qu6 C 40-48 disorder

Region 3qu6 C 49-110 order

Region 3qu6 C 111-113 disorder

Evidence X-RAY 3qu6 B Reference

Region 3qu6 B 1-4 disorder

Region 3qu6 B 5-40 order

Region 3qu6 B 41-48 disorder

Region 3qu6 B 49-110 order

Region 3qu6 B 111-113 disorder

Evidence X-RAY 3qu6 A Reference

Region 3qu6 A 1-4 disorder

Region 3qu6 A 5-40 order

Region 3qu6 A 41-48 disorder

Region 3qu6 A 49-110 order

Region 3qu6 A 111-113 disorder

Seq 1-113 Homo tetramer :

Evidence X-RAY 2pi0 D Reference

Region 2pi0 D 1-2 disorder

Region 2pi0 D 3-46 order

Region 2pi0 D 47-51 disorder

Region 2pi0 D 52-111 order

Region 2pi0 D 112-113 disorder

Evidence X-RAY 2pi0 C Reference

Region 2pi0 C 1-3 disorder

Region 2pi0 C 4-110 order

Region 2pi0 C 111-113 disorder

Evidence X-RAY 2pi0 B Reference

Region 2pi0 B 1-112 order

Region 2pi0 B 113-113 disorder

Evidence X-RAY 2pi0 A Reference

Region 2pi0 A 1-4 disorder

Region 2pi0 A 5-43 order

Region 2pi0 A 44-51 disorder

Region 2pi0 A 52-110 order

Region 2pi0 A 111-113 disorder

Seq 1-123 Homo tetramer :

Evidence X-RAY 2o6g H Reference

Region 2o6g H 1-3 disorder

Region 2o6g H 4-111 order

Region 2o6g H 112-123 disorder

Evidence X-RAY 2o6g G Reference

Region 2o6g G 1-2 disorder

Region 2o6g G 3-112 order

Region 2o6g G 113-123 disorder

Evidence X-RAY 2o6g F Reference

Region 2o6g F 1-3 disorder

Region 2o6g F 4-111 order

Region 2o6g F 112-123 disorder

Evidence X-RAY 2o6g E Reference

Region 2o6g E 1-2 disorder

Region 2o6g E 3-112 order

Region 2o6g E 113-123 disorder

Seq 9-111 Hetero dimer : IID00207Complex,IID00491Complex,IID00710Complex

Evidence X-RAY 2o61 A Reference

Region 2o61 A 9-111 order

Seq 173-427 Homo dimer :

Evidence X-RAY 1qwt B Reference

Region 1qwt B 173-188 disorder

Region 1qwt B 189-427 order

Evidence X-RAY 1qwt A Reference

Region 1qwt A 173-188 disorder

Region 1qwt A 189-427 order

Seq 175-427 Monomer :

Evidence X-RAY 1j2f B Reference

Region 1j2f B 175-188 disorder

Region 1j2f B 189-233 order

Region 1j2f B 234-236 disorder

Region 1j2f B 237-422 order

Region 1j2f B 423-427 disorder

Evidence X-RAY 1j2f A Reference

Region 1j2f A 175-189 disorder

Region 1j2f A 190-230 order

Region 1j2f A 231-237 disorder

Region 1j2f A 238-422 order

Region 1j2f A 423-427 disorder

Evidence X-RAY 3a77 D Reference

Region 3a77 D 189-189 disorder

Region 3a77 D 190-230 order

Region 3a77 D 231-237 disorder

Region 3a77 D 238-426 order

Region 3a77 D 427-427 disorder

Evidence X-RAY 3a77 C Reference

Region 3a77 C 189-231 order

Region 3a77 C 232-236 disorder

Region 3a77 C 237-426 order

Region 3a77 C 427-427 disorder

Evidence X-RAY 3a77 B Reference

Region 3a77 B 189-232 order

Region 3a77 B 233-236 disorder

Region 3a77 B 237-426 order

Region 3a77 B 427-427 disorder

Evidence X-RAY 3a77 A Reference

Region 3a77 A 189-229 order

Region 3a77 A 230-236 disorder

Region 3a77 A 237-427 order

Seq 189-398 Hetero tetramer : IID00092Complex

Evidence X-RAY 5jem B Reference

Region 5jem B 189-195 disorder

Region 5jem B 196-398 order

Evidence X-RAY 5jem A Reference

Region 5jem A 189-195 disorder

Region 5jem A 196-398 order

Seq 189-398 Hetero tetramer : IID00092Complex

Evidence X-RAY 5jem G Reference

Region 5jem G 189-198 disorder

Region 5jem G 199-398 order

Evidence X-RAY 5jem E Reference

Region 5jem E 189-195 disorder

Region 5jem E 196-398 order

Seq 189-427 Hetero trimer : Q86WV6

Evidence X-RAY 5jej B Reference

Region 5jej B 189-421 order

Region 5jej B 422-427 disorder

Evidence X-RAY 5jej A Reference

Region 5jej A 189-422 order

Region 5jej A 423-427 disorder

Seq 189-427 Hetero dimer : Q8IUC6

Evidence X-RAY 5jel A Reference

Region 5jel A 189-422 order

Region 5jel A 423-427 disorder

Seq 189-427 Hetero dimer : Q7Z434

Evidence X-RAY 5jek B Reference

Region 5jek B 189-422 order

Region 5jek B 423-427 disorder

Evidence X-RAY 5jek A Reference

Region 5jek A 189-422 order

Region 5jek A 423-427 disorder

Seq 189-427 Hetero dimer : Q99FX5

Evidence X-RAY 5jer G Reference

Region 5jer G 189-422 order

Region 5jer G 423-427 disorder

Evidence X-RAY 5jer E Reference

Region 5jer E 189-422 order

Region 5jer E 423-427 disorder

Evidence X-RAY 5jer C Reference

Region 5jer C 189-422 order

Region 5jer C 423-427 disorder

Evidence X-RAY 5jer A Reference

Region 5jer A 189-422 order

Region 5jer A 423-427 disorder

Evidence X-RAY 5jeo A Reference

Region 5jeo A 189-422 order

Region 5jeo A 423-427 disorder

Seq 196-386 Hetero dimer : IID00092Complex

Evidence X-RAY 1zoq B Reference

Region 1zoq B 196-386 order

Evidence X-RAY 1zoq A Reference

Region 1zoq A 196-386 order

Seqphosphorylation

3-3 Phosphothreonine

14-14 Phosphoserine

75-75 Phosphothreonine

97-97 Phosphoserine

123-123 Phosphoserine

175-175 (Microbial infection) Phosphoserine

180-180 Phosphothreonine

188-188 Phosphoserine

237-237 Phosphothreonine

244-244 Phosphothreonine

253-253 Phosphothreonine

385-385 Phosphoserine

386-386 Phosphoserine; by TBK1

396-396 Phosphoserine; by IKKE and TBK1

398-398 Phosphoserine

404-404 Phosphothreonine

427-427 Phosphoserine

Prediction

NeProc

Disorder 1-5,111-199,298-309,383-399,409-427

Order 6-110,200-297,310-382,400-408

ProS 111-120,127-137,156-161,191-199,306-309,383-399,409-423

AlphaFold

Disorder 1-3,111-188,232-234,250-254,423-427

Order 4-110,189-231,235-249,255-422

Pfam Hmmer

PF00605 7-112 9e-21

Function

Function in SwissProt

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed:22394562, PubMed:25636800, PubMed:27302953). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:11846977, PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:32972995). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed:16846591, PubMed:16979567, PubMed:20049431). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed:22394562, PubMed:25636800, PubMed:27302953). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed:16154084, PubMed:27302953, PubMed:33440148). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed:16846591). In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis (PubMed:25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).

Biological Process

Diagram with PDB data
IRF3/DNA	Crystal Structure of IRF-3 bound to the PRDIII-I regulatory element of the human interferon-B enhancer

See also
Diagram with PDB data
CREBBP/IRF3	Crystal structure of human phosphorylated IRF-3 bound to CBP