IDEAL List

IDEAL

IID00246
UniprotQ14191
ProteinWerner syndrome ATP-dependent helicase
GeneWRN
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS xml

Structure

Experiment

:order disorder conflict PDB cluster ProS Pfam Domain SEG

1432

order/disorder by at least rule

disorder by at least rule

order by at least rule

order/disorder by majority rule

Seq 38-236 Monomer :

Evidence X-RAY 2fc0 A Reference

Region 2fc0 A 38-231 order

Region 2fc0 A 232-236 disorder

Evidence X-RAY 2fby A Reference

Region 2fby A 38-231 order

Region 2fby A 232-236 disorder

Evidence X-RAY 2fbx A Reference

Region 2fbx A 38-231 order

Region 2fbx A 232-236 disorder

Evidence X-RAY 2fbv A Reference

Region 2fbv A 38-230 order

Region 2fbv A 231-236 disorder

Evidence X-RAY 2fbt A Reference

Region 2fbt A 38-231 order

Region 2fbt A 232-236 disorder

Seq 949-1079 Homo dimer :

Evidence X-RAY 3aaf B Reference

Region 3aaf B 949-955 disorder

Region 3aaf B 956-1064 order

Region 3aaf B 1065-1079 disorder

Evidence X-RAY 3aaf A Reference

Region 3aaf A 949-955 disorder

Region 3aaf A 956-1064 order

Region 3aaf A 1065-1079 disorder

Seq 949-1092 Monomer :

Evidence NMR 2axl A Reference

Region 2axl A 949-954 disorder

Region 2axl A 949-953 high_rmsd

Region 2axl A 955-1068 order

Region 2axl A 1069-1092 disorder

Region 2axl A 1073-1092 high_rmsd

Seq 1140-1242 Monomer :

Evidence NMR 2dgz A Reference

Region 2dgz A 1140-1239 order

Region 2dgz A 1140-1141 high_rmsd

Region 2dgz A 1235-1239 high_rmsd

Evidence X-RAY 2e1f A Reference

Region 2e1f A 1142-1235 order

Region 2e1f A 1236-1242 disorder

Evidence X-RAY 2e1e A Reference

Region 2e1e A 1142-1235 order

Region 2e1e A 1236-1242 disorder

Seqphosphorylation

426-426 Phosphoserine

440-440 Phosphoserine

453-453 Phosphoserine

467-467 Phosphoserine

478-478 Phosphoserine

1133-1133 Phosphoserine

1400-1400 Phosphoserine

Seqacetylation

2-2 N-acetylserine

Prediction

NeProc

Disorder 1-30,305-529,1066-1085,1232-1251,1362-1432

Order 31-304,530-923,929-948,953-1065,1086-1137,1151-1231,1256-1361

ProS 13-30,309-318,325-379,384-419,427-505,525-529,1066-1074,1371-1376,1406-1414,1428-1432

AlphaFold

Disorder 1-42,88-94,231-251,253-254,257-258,261-262,264-282,292-528,621-625,677-678,800-800,823-827,919-919,921-927,948-956,1065-1065,1067-1067,1071-1145,1147-1147,1234-1254,1357-1432

Order 43-87,95-230,252-252,255-256,259-260,263-263,283-291,529-620,626-676,679-799,801-822,828-918,920-920,928-947,957-1064,1066-1066,1068-1070,1146-1146,1148-1233,1255-1356

Pfam Hmmer

PF01612 57-231 4.6e-55

PF00270 550-714 2.8e-28

PF00271 783-859 7.4e-26

PF00570 1150-1229 2.7e-27

SEG 399-414 ,506-526 ,1071-1080 ,1117-1129

Function

Function in SwissProt

Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation.