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IID00314
 UniprotQ00403
ProteinTranscription initiation factor IIB
GeneGTF2B
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
316
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 1-60 Monomer :
 Evidence NMR 1ro4 A Reference
       Region 1ro4 A 1-1 disorder
       Region 1ro4 A 2-59 order
       Region 1ro4 A 2-11 high_rmsd
       Region 1ro4 A 44-59 high_rmsd
       Region 1ro4 A 60-60 disorder
 Evidence NMR 1rly A Reference
       Region 1rly A 1-1 disorder
       Region 1rly A 2-59 order
       Region 1rly A 2-11 high_rmsd
       Region 1rly A 45-59 high_rmsd
       Region 1rly A 60-60 disorder
 Evidence NMR 1dl6 A Reference
       Region 1dl6 A 2-59 order
       Region 1dl6 A 2-12 high_rmsd
       Region 1dl6 A 28-29 high_rmsd
       Region 1dl6 A 44-59 high_rmsd
Seq 107-316 Monomer :
 Evidence NMR 1tfb A Reference
       Region 1tfb A 112-316 order
 Evidence X-RAY 5wh1 D Reference
       Region 5wh1 D 107-316 order
 Evidence X-RAY 5wh1 C Reference
       Region 5wh1 C 107-316 order
 Evidence X-RAY 5wh1 B Reference
       Region 5wh1 B 107-316 order
 Evidence X-RAY 5wh1 A Reference
       Region 5wh1 A 107-107 disorder
       Region 5wh1 A 108-208 order
       Region 5wh1 A 209-211 disorder
       Region 5wh1 A 212-316 order
Seq 110-316 Hetero dimer : IID00367Complex
 Evidence X-RAY 1c9b Q Reference
       Region 1c9b Q 110-316 order
 Evidence X-RAY 1c9b M Reference
       Region 1c9b M 110-316 order
 Evidence X-RAY 1c9b I Reference
       Region 1c9b I 110-316 order
 Evidence X-RAY 1c9b E Reference
       Region 1c9b E 110-316 order
 Evidence X-RAY 1c9b A Reference
       Region 1c9b A 110-316 order
Seq 112-316 Hetero dimer : IID90007Complex
 Evidence NMR 2phg A Reference
       Region 2phg A 112-316 order
Seq 113-316 Hetero dimer : P28147
 Evidence X-RAY 1vol A Reference
       Region 1vol A 113-316 order
Seqphosphorylation
    92-92 Phosphoserine
    76-76 Phosphoserine
    70-70 Phosphoserine
Seqacetylation
    238-238 N6-acetyllysine; by autocatalysis
 
Prediction
NeProc
Disorder 1-10,61-65,89-107
Order 11-60,66-88,108-316
ProS 7-10,61-65,89-107
AlphaFold
Disorder 1-11,52-63,104-106
Order 12-51,64-103,107-316
Pfam Hmmer
PF08271 13-55 3.3e-18
PF00382 121-191 3e-31
PF00382 215-285 4.2e-33
SEG 252-266
Function
Function in SwissProt
General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352). Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841). Modulates transcription start site selection (PubMed:10318856). Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).