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IID00340
 UniprotQ99708
ProteinDNA endonuclease RBBP8
GeneRBBP8
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
897
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 18-52 Homo tetramer :
 Evidence X-RAY 4d2h A Reference
       Region 4d2h A 18-51 order
       Region 4d2h A 52-52 disorder
 Evidence X-RAY 4d2h B Reference
       Region 4d2h B 18-49 order
       Region 4d2h B 50-52 disorder
 Evidence X-RAY 4d2h C Reference
       Region 4d2h C 18-18 disorder
       Region 4d2h C 19-52 order
 Evidence X-RAY 4d2h D Reference
       Region 4d2h D 18-18 disorder
       Region 4d2h D 19-48 order
       Region 4d2h D 49-52 disorder
 Evidence X-RAY 4d2h E Reference
       Region 4d2h E 18-18 disorder
       Region 4d2h E 19-48 order
       Region 4d2h E 49-52 disorder
 Evidence X-RAY 4d2h F Reference
       Region 4d2h F 18-52 order
 Evidence X-RAY 4d2h G Reference
       Region 4d2h G 18-52 order
 Evidence X-RAY 4d2h H Reference
       Region 4d2h H 18-18 disorder
       Region 4d2h H 19-51 order
       Region 4d2h H 52-52 disorder
Seq 641-685 Monomer : IID00310Complex
 Evidence NMR 2l4z A Reference
       Region 2l4z A 641-685 order
       Region 2l4z A 641-661 high_rmsd
       Region 2l4z A 678-685 high_rmsd
Seqphosphorylation
    723-723 Phosphoserine
    847-847 Phosphothreonine; by CDK1
    745-745 Phosphoserine; by ATM
    679-679 Phosphoserine
    664-664 Phosphoserine; by ATM
    379-379 Phosphoserine
    349-349 Phosphoserine
    327-327 Phosphoserine
    326-326 Phosphoserine
    315-315 Phosphothreonine; by CDK2
    276-276 Phosphoserine
    233-233 Phosphoserine
 
Prediction
NeProc
Disorder 1-16,155-786,878-897
Order 17-154,787-877
ProS 5-12,159-163,182-189,200-207,219-267,300-368,382-447,455-529,536-545,551-593,601-616,631-646,652-674,680-695,707-786,881-894
AlphaFold
Disorder 1-16,148-663,665-723,727-797,843-846,870-897
Order 17-147,664-664,724-726,798-842,847-869
SEG 443-455
Function
Function in SwissProt
Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway (PubMed:17965729, PubMed:19202191, PubMed:19759395, PubMed:20064462). HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse (PubMed:17965729, PubMed:19202191). Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ) (PubMed:19202191). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA (PubMed:16581787, PubMed:17965729, PubMed:19759395, PubMed:20064462). Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage (PubMed:15485915, PubMed:16818604). During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations (By similarity).