IDEAL List

IDEAL

IID00384
UniprotQ14145
ProteinKelch-like ECH-associated protein 1
GeneKEAP1
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS Network xml rdf

Structure

Experiment

:order disorder conflict PDB cluster ProS Pfam Domain SEG

624

order/disorder by at least rule

disorder by at least rule

order by at least rule

order/disorder by majority rule

Seq 48-180 Monomer :

Evidence X-RAY 5git A Reference

Region 5git A 48-50 disorder

Region 5git A 51-180 order

Evidence X-RAY 4cxi A Reference

Region 4cxi A 48-49 disorder

Region 4cxi A 50-179 order

Region 4cxi A 180-180 disorder

Seq 48-182 Homo dimer :

Evidence X-RAY 5daf A Reference

Region 5daf A 49-49 disorder

Region 5daf A 50-112 order

Region 5daf A 113-119 disorder

Region 5daf A 120-177 order

Region 5daf A 178-182 disorder

Evidence X-RAY 5dad A Reference

Region 5dad A 49-49 disorder

Region 5dad A 50-112 order

Region 5dad A 113-119 disorder

Region 5dad A 120-179 order

Region 5dad A 180-182 disorder

Evidence X-RAY 4cxt A Reference

Region 4cxt A 48-48 disorder

Region 4cxt A 49-180 order

Evidence X-RAY 4cxj A Reference

Region 4cxj A 48-48 disorder

Region 4cxj A 49-179 order

Region 4cxj A 180-180 disorder

Seq 51-204 Hetero dimer : Q13618

Evidence X-RAY 5nlb A Reference

Region 5nlb A 51-204 order

Seq 321-609 Homo dimer :

Evidence X-RAY 3zgd B Reference

Region 3zgd B 321-324 disorder

Region 3zgd B 325-609 order

Evidence X-RAY 3zgd A Reference

Region 3zgd A 321-324 disorder

Region 3zgd A 325-609 order

Evidence X-RAY 4l7b B Reference

Region 4l7b B 321-609 order

Evidence X-RAY 4l7b A Reference

Region 4l7b A 321-321 disorder

Region 4l7b A 322-609 order

Seq 321-609 Hetero dimer :

Evidence X-RAY 5x54 B Reference

Region 5x54 B 321-324 disorder

Region 5x54 B 325-609 order

Seq 321-609 Homo trimer :

Evidence X-RAY 4n1b C Reference

Region 4n1b C 321-324 disorder

Region 4n1b C 325-609 order

Evidence X-RAY 4n1b B Reference

Region 4n1b B 321-326 disorder

Region 4n1b B 327-609 order

Evidence X-RAY 4n1b A Reference

Region 4n1b A 321-325 disorder

Region 4n1b A 326-609 order

Evidence X-RAY 4l7d C Reference

Region 4l7d C 321-324 disorder

Region 4l7d C 325-609 order

Evidence X-RAY 4l7d B Reference

Region 4l7d B 321-325 disorder

Region 4l7d B 326-609 order

Evidence X-RAY 4l7d A Reference

Region 4l7d A 321-325 disorder

Region 4l7d A 326-609 order

Evidence X-RAY 4l7c C Reference

Region 4l7c C 321-325 disorder

Region 4l7c C 326-609 order

Evidence X-RAY 4l7c B Reference

Region 4l7c B 321-325 disorder

Region 4l7c B 326-609 order

Evidence X-RAY 4l7c A Reference

Region 4l7c A 321-325 disorder

Region 4l7c A 326-609 order

Seq 321-609 Monomer :

Evidence X-RAY 1zgk A Reference

Region 1zgk A 321-321 disorder

Region 1zgk A 322-609 order

Evidence X-RAY 4xmb A Reference

Region 4xmb A 321-323 disorder

Region 4xmb A 324-609 order

Evidence X-RAY 3vnh A Reference

Region 3vnh A 321-321 disorder

Region 3vnh A 322-609 order

Evidence X-RAY 3vng A Reference

Region 3vng A 321-321 disorder

Region 3vng A 322-609 order

Evidence X-RAY 1u6d X Reference

Region 1u6d X 321-321 disorder

Region 1u6d X 322-609 order

Evidence X-RAY 4iqk A Reference

Region 4iqk A 321-324 disorder

Region 4iqk A 325-609 order

Evidence X-RAY 4in4 C Reference

Region 4in4 C 321-609 order

Evidence X-RAY 4in4 B Reference

Region 4in4 B 321-325 disorder

Region 4in4 B 326-609 order

Evidence X-RAY 4in4 A Reference

Region 4in4 A 321-321 disorder

Region 4in4 A 322-609 order

Evidence X-RAY 4ifn X Reference

Region 4ifn X 321-324 disorder

Region 4ifn X 325-609 order

Evidence X-RAY 4ifj A Reference

Region 4ifj A 321-321 disorder

Region 4ifj A 322-609 order

Seq 321-609 Hetero dimer : IID00213Complex

Evidence X-RAY 4ifl X Reference

Region 4ifl X 321-324 disorder

Region 4ifl X 325-609 order

Evidence X-RAY 2flu X Reference

Region 2flu X 321-324 disorder

Region 2flu X 325-609 order

Seq 321-609 Hetero dimer :

Evidence X-RAY 5x54 A Reference

Region 5x54 A 321-324 disorder

Region 5x54 A 325-609 order

Seq 321-609 Hetero trimer : IID00213Complex

Evidence X-RAY 3zgc A Reference

Region 3zgc A 321-324 disorder

Region 3zgc A 325-609 order

Evidence X-RAY 3zgc B Reference

Region 3zgc B 321-324 disorder

Region 3zgc B 325-609 order

Seq 321-611 Hetero tetramer :

Evidence X-RAY 5f72 C Reference

Region 5f72 C 321-324 disorder

Region 5f72 C 325-609 order

Region 5f72 C 610-611 disorder

Evidence X-RAY 5f72 K Reference

Region 5f72 K 321-324 disorder

Region 5f72 K 325-609 order

Region 5f72 K 610-611 disorder

Prediction

NeProc

Disorder 1-49,620-624

Order 50-619

ProS 15-21,620-624

AlphaFold

Disorder 1-50,314-318,620-624

Order 51-313,319-619

Pfam Hmmer

PF00651 67-179 4.5e-39

PF07707 184-286 8.9e-46

PF01344 315-359 1.2e-05

PF01344 361-410 1.1e-15

PF01344 412-457 2.3e-14

PF01344 459-504 1.5e-15

PF01344 506-551 9.3e-19

PF01344 553-598 4.9e-18

Function

Function in SwissProt

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination (PubMed:14585973, PubMed:15379550, PubMed:15572695, PubMed:15983046, PubMed:15601839). KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes (PubMed:15601839, PubMed:16006525). In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes (PubMed:19489739, PubMed:16006525, PubMed:17127771, PubMed:18251510, PubMed:29590092). In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2 (PubMed:20452972). The BCR(KEAP1) complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation (PubMed:28380357). The BCR(KEAP1) complex also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome (PubMed:15379550, PubMed:17046835).