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IID00417
 UniprotQ8NG31
ProteinKinetochore scaffold 1
GeneKNL1
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
 Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
2342
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 176-226 Hetero dimer : IID00406Complex
 Evidence X-RAY 4a1g H Reference
       Region 4a1g H 176-200 disorder
       Region 4a1g H 201-216 order
       Region 4a1g H 217-226 disorder
 Evidence X-RAY 4a1g G Reference
       Region 4a1g G 176-200 disorder
       Region 4a1g G 201-215 order
       Region 4a1g G 216-226 disorder
 Evidence X-RAY 4a1g F Reference
       Region 4a1g F 176-200 disorder
       Region 4a1g F 201-215 order
       Region 4a1g F 216-226 disorder
 Evidence X-RAY 4a1g E Reference
       Region 4a1g E 176-201 disorder
       Region 4a1g E 202-213 order
       Region 4a1g E 214-226 disorder
Seq 234-252 Hetero dimer : IID00407Complex
 Evidence X-RAY 3si5 Y Reference
       Region 3si5 Y 234-237 disorder
       Region 3si5 Y 238-250 order
       Region 3si5 Y 251-252 disorder
 Evidence X-RAY 3si5 X Reference
       Region 3si5 X 234-251 order
       Region 3si5 X 252-252 disorder
Seqdisorder 243-252
 Evidence CD Reference
       Region 243-252 disorder
Seq 2117-2337 Hetero dimer : Q96IY1
 Evidence X-RAY 4nf9 B Reference
       Region 4nf9 B 2117-2130 disorder
       Region 4nf9 B 2131-2337 order
 Evidence X-RAY 4nf9 A Reference
       Region 4nf9 A 2117-2121 disorder
       Region 4nf9 A 2122-2337 order
Seq 2131-2337 Monomer :
 Evidence X-RAY 4nfa A Reference
       Region 4nfa A 2131-2213 order
       Region 4nfa A 2214-2216 disorder
       Region 4nfa A 2217-2337 order
SeqProS possible 201-216 This region is described to be disordered in the free state (PubMed=22331848). Hetero dimer : IID00406Complex
       Region 4a1g F 201-215 order
       Region 4a1g G 201-215 order
       Region 4a1g H 201-216 order
SeqProS verified 234-251 Hetero dimer : IID00407Complex
       Region 3si5 X 234-251 order
       Region 3si5 Y 238-250 order
       Region 243-252 disorder
Seqphosphorylation
    586-586 Phosphothreonine
    1773-1773 Phosphoserine
    1675-1675 Phosphoserine
    1448-1448 Phosphoserine
    1088-1088 Phosphoserine
    1076-1076 Phosphoserine
    1039-1039 Phosphoserine
    956-956 Phosphoserine
    767-767 Phosphoserine
    1831-1831 Phosphoserine
    584-584 Phosphoserine
    578-578 Phosphoserine
    539-539 Phosphothreonine
    60-60 Phosphoserine
    32-32 Phosphoserine
    1860-1860 Phosphoserine
    1845-1845 Phosphoserine
 
Prediction
NeProc
Disorder 1-120,200-292,297-308,318-447,453-466,475-1181,1189-1900,1919-1927
Order 121-124,129-199,293-296,309-317,448-452,467-474,1182-1188,1901-1918,1928-2342
ProS 1-50,56-80,88-106,114-120,200-250,257-267,284-292,297-308,318-326,333-355,361-379,387-393,399-432,453-466,475-483,500-524,544-550,558-577,583-625,643-648,695-716,724-731,741-748,756-802,811-816,838-862,874-891,897-912,928-958,965-990,995-1001,1021-1035,1062-1076,1095-1128,1140-1167,1181-1181,1189-1206,1224-1260,1268-1284,1289-1292,1299-1328,1354-1379,1394-1414,1432-1452,1474-1490,1495-1508,1528-1565,1573-1592,1613-1619,1642-1651,1662-1709,1721-1747,1753-1774,1788-1818,1826-1829,1854-1900
AlphaFold
Disorder 1-1882,1907-1926,2061-2074,2123-2124,2126-2133,2162-2174,2210-2211,2337-2342
Order 1883-1906,1927-2060,2075-2122,2125-2125,2134-2161,2175-2209,2212-2336
SEG 622-640 ,1084-1097 ,1605-1615 ,1841-1853 ,2082-2103 ,2252-2263
Function
Function in SwissProt
Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Required for attachment of the kinetochores to the spindle microtubules. Directly links BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.