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IID00641
UniprotO00267
ProteinTranscription elongation factor SPT5
GeneSUPT5H
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
1087
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 176-273 Hetero dimer : P63272
 Evidence X-RAY 3h7h B Reference
       Region 3h7h B 176-269 order
       Region 3h7h B 270-273 disorder
Seq 420-471 Monomer :
 Evidence NMR 2e6z A Reference
       Region 2e6z A 420-471 order
Seq 462-523 Monomer :
 Evidence NMR 2do3 A Reference
       Region 2do3 A 462-523 order
       Region 2do3 A 462-468 high_rmsd
Seq 536-646 Homo dimer :
 Evidence X-RAY 5oho A Reference
       Region 5oho A 536-646 order
 Evidence X-RAY 5oho B Reference
       Region 5oho B 536-646 order
Seq 694-757 Monomer :
 Evidence NMR 2e70 A Reference
       Region 2e70 A 694-757 order
       Region 2e70 A 694-699 high_rmsd
       Region 2e70 A 756-757 high_rmsd
Seq 778-790 Hetero decamer : Q92541
 Evidence X-RAY 4l1u J Reference
       Region 4l1u J 778-781 disorder
       Region 4l1u J 782-786 order
       Region 4l1u J 787-790 disorder
 Evidence X-RAY 4l1u I Reference
       Region 4l1u I 778-782 disorder
       Region 4l1u I 783-787 order
       Region 4l1u I 788-790 disorder
 Evidence X-RAY 4l1u H Reference
       Region 4l1u H 778-779 disorder
       Region 4l1u H 780-790 order
 Evidence X-RAY 4l1u G Reference
       Region 4l1u G 778-780 disorder
       Region 4l1u G 781-788 order
       Region 4l1u G 789-790 disorder
Seq 979-1087 Monomer :
 Evidence X-RAY 5ohq A Reference
       Region 5ohq A 979-1086 order
       Region 5ohq A 1087-1087 disorder
Seq 980-988 Hetero hexamer : P18465
 Evidence X-RAY 5u98 C Reference
       Region 5u98 C 980-988 order
 Evidence X-RAY 5u98 F Reference
       Region 5u98 F 980-988 order
SeqProS predicted 780-788 The unbound state of this region is predicted to be disordered by DICHOT and MobiDB. Hetero decamer : Q92541
       Region 4l1u G 781-788 order
       Region 4l1u H 780-790 order
       Region 4l1u I 783-787 order
       Region 4l1u J 782-786 order
Seqphosphorylation
    1034-1034 Phosphothreonine
    804-804 Phosphoserine
    806-806 Phosphothreonine
    791-791 Phosphothreonine
    784-784 Phosphothreonine; by CDK9
    789-789 Phosphoserine
    775-775 Phosphothreonine; by CDK9
    686-686 Phosphoserine
    36-36 Phosphoserine
    666-666 Phosphoserine
    32-32 Phosphoserine
Seqacetylation
    718-718 N6-acetyllysine
 
Prediction
NeProc
Disorder 1-32,61-161,566-576,652-703,757-920,932-937
Order 33-60,162-565,577-651,704-756,921-931,938-1087
ProS 4-8,71-81,97-161,566-576,652-655,660-685,692-703,757-920,932-937
AlphaFold
Disorder 1-137,140-140,144-163,267-272,320-320,322-322,325-326,328-332,397-416,484-484,531-531,579-580,648-702,754-977,1086-1087
Order 138-139,141-143,164-266,273-319,321-321,323-324,327-327,333-396,417-483,485-530,532-578,581-647,703-753,978-1085
Pfam Hmmer
PF03439 139-188 3e-26
PF03439 224-272 5e-23
PF00467 273-306 0.00067
PF00467 420-451 9.6e-05
PF00467 472-503 4.2e-08
PF00467 594-627 2.4e-05
PF00467 704-737 7.1e-06
SEG 2-25 ,37-64 ,78-94 ,678-701 ,832-843 ,896-911
Function
Function in SwissProt
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
Biological Process
Diagram with PDB data
SUPT5H/RTF1Crystal Structure of Human Rtf1 Plus3 Domain in Complex with Spt5 CTR Phosphopeptide