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IID00660
UniprotP54619
Protein5'-AMP-activated protein kinase subunit gamma-1
GenePRKAG1
OrganismHomo sapiens
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
331
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 1-331 Hetero hexamer : IID00661Complex,IID00686Complex
 Evidence X-RAY 5iso F Reference
       Region 5iso F 1-26 disorder
       Region 5iso F 27-325 order
       Region 5iso F 326-331 disorder
 Evidence X-RAY 5iso E Reference
       Region 5iso E 1-26 disorder
       Region 5iso E 27-324 order
       Region 5iso E 325-331 disorder
Seq 1-331 Hetero trimer : IID00661Complex,IID00686Complex
 Evidence X-RAY 4zhx F Reference
       Region 4zhx F 2-25 disorder
       Region 4zhx F 26-325 order
       Region 4zhx F 326-331 disorder
 Evidence X-RAY 4zhx E Reference
       Region 4zhx E 2-26 disorder
       Region 4zhx E 27-324 order
       Region 4zhx E 325-331 disorder
 Evidence X-RAY 4cff E Reference
       Region 4cff E 1-26 disorder
       Region 4cff E 27-325 order
       Region 4cff E 326-331 disorder
 Evidence X-RAY 4cff F Reference
       Region 4cff F 1-26 disorder
       Region 4cff F 27-121 order
       Region 4cff F 122-127 disorder
       Region 4cff F 128-324 order
       Region 4cff F 325-331 disorder
 Evidence X-RAY 4cfe E Reference
       Region 4cfe E 1-26 disorder
       Region 4cfe E 27-122 order
       Region 4cfe E 123-127 disorder
       Region 4cfe E 128-325 order
       Region 4cfe E 326-331 disorder
 Evidence X-RAY 4cfe F Reference
       Region 4cfe F 1-26 disorder
       Region 4cfe F 27-121 order
       Region 4cfe F 122-127 disorder
       Region 4cfe F 128-270 order
       Region 4cfe F 271-275 disorder
       Region 4cfe F 276-325 order
       Region 4cfe F 326-331 disorder
Seq 2-331 Hetero trimer : IID00661Complex,IID00686Complex,Q13131
 Evidence X-RAY 5ezv F Reference
       Region 5ezv F 2-24 disorder
       Region 5ezv F 25-324 order
       Region 5ezv F 325-331 disorder
Seq 2-331 Hetero trimer : IID00661Complex,IID00686Complex,Q13131
 Evidence X-RAY 5ezv E Reference
       Region 5ezv E 2-23 disorder
       Region 5ezv E 24-123 order
       Region 5ezv E 124-124 disorder
       Region 5ezv E 125-326 order
       Region 5ezv E 327-331 disorder
Seq 24-327 Hetero trimer : O43741,Q13131
 Evidence X-RAY 4rer G Reference
       Region 4rer G 24-24 disorder
       Region 4rer G 25-324 order
       Region 4rer G 325-327 disorder
Seq 24-327 Hetero trimer : O43741,Q13131
 Evidence X-RAY 4rew G Reference
       Region 4rew G 24-24 disorder
       Region 4rew G 25-325 order
       Region 4rew G 326-327 disorder
Seq 182-325 Homo dimer :
 Evidence X-RAY 2uv7 A Reference
       Region 2uv7 A 182-323 order
       Region 2uv7 A 324-325 disorder
 Evidence X-RAY 2uv6 A Reference
       Region 2uv6 A 182-323 order
       Region 2uv6 A 324-325 disorder
 Evidence X-RAY 2uv5 A Reference
       Region 2uv5 A 182-323 order
       Region 2uv5 A 324-325 disorder
 Evidence X-RAY 2uv4 A Reference
       Region 2uv4 A 182-323 order
       Region 2uv4 A 324-325 disorder
Seqphosphorylation
    261-261 Phosphoserine; by ULK1
    263-263 Phosphothreonine; by ULK1
    270-270 Phosphoserine; by ULK1
 
Prediction
NeProc
Disorder 1-22,328-331
Order 23-327
AlphaFold
Disorder 1-24,101-110,121-126,270-275,327-327,329-331
Order 25-100,111-120,127-269,276-326,328-328
Pfam Hmmer
PF00571 42-177 7.8e-18
PF00571 198-323 2e-21
Function
Function in SwissProt
AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
Biological Process
See also
Diagram with PDB data
PRKAA2/PRKAB1/PRKAG1Structure of full length human AMPK in complex with a small molecule activator, a benzimidazole derivative (991)