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IID50021
UniprotP32776
ProteinGeneral transcription and DNA repair factor IIH subunit TFB1
GeneTFB1
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c)
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
642
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 1-115 Monomer :
 Evidence NMR 1y5o A Reference
       Region 1y5o A 1-115 order
       Region 1y5o A 68-82 high_rmsd
Seq 1-115 Hetero dimer : P21698
 Evidence NMR 2n0y A Reference
       Region 2n0y A 1-115 order
       Region 2n0y A 70-79 high_rmsd
Seq 1-115 Hetero dimer : IID90006Complex
 Evidence NMR 2k2u A Reference
       Region 2k2u A 1-115 order
       Region 2k2u A 33-35 high_rmsd
       Region 2k2u A 69-80 high_rmsd
Seq 1-115 Hetero dimer : IID00015Complex
 Evidence NMR 2gs0 A Reference
       Region 2gs0 A 1-115 order
       Region 2gs0 A 73-80 high_rmsd
Seq 1-115 Hetero dimer : IID90021Complex
 Evidence NMR 2mkr A Reference
       Region 2mkr A 1-115 order
       Region 2mkr A 1-1 high_rmsd
       Region 2mkr A 69-80 high_rmsd
       Region 2mkr A 115-115 high_rmsd
Seq 2-115 Hetero dimer : IID00468Complex
 Evidence NMR 2n23 A Reference
       Region 2n23 A 2-115 order
       Region 2n23 A 69-81 high_rmsd
Seq 2-115 Hetero dimer : IID50166Complex
 Evidence NMR 2lox A Reference
       Region 2lox A 2-115 order
       Region 2lox A 69-80 high_rmsd
Seq 2-115 Hetero dimer : IID00207Complex
 Evidence NMR 5urn A Reference
       Region 5urn A 2-115 order
       Region 5urn A 73-79 high_rmsd
       Region 5urn A 115-115 high_rmsd
Seq 2-115 Hetero dimer : IID50167Complex
 Evidence NMR 2m14 A Reference
       Region 2m14 A 2-115 order
       Region 2m14 A 69-80 high_rmsd
Seq 2-115 Hetero dimer : IID00468Complex
 Evidence NMR 2l2i A Reference
       Region 2l2i A 2-115 order
       Region 2l2i A 34-34 high_rmsd
       Region 2l2i A 67-80 high_rmsd
Seqphosphorylation
    150-150 Phosphothreonine
 
Prediction
NeProc
Disorder 1-4,74-84,114-169,225-232,377-460,512-538,637-642
Order 5-67,85-113,170-224,233-376,465-511,539-636
ProS 225-232,377-389,411-447,457-460,512-533,637-642
AlphaFold
Disorder 1-1,67-82,126-168,238-250,300-307,317-321,323-357,360-361,363-369,390-412,414-415,438-465,469-469,479-493,514-542,641-642
Order 2-66,83-125,169-237,251-299,308-316,322-322,358-359,362-362,370-389,413-413,416-437,466-468,470-478,494-513,543-640
Pfam Hmmer
PF08567 1-76 5e-34
PF03909 161-227 7.2e-16
PF03909 236-301 4.1e-18
SEG 164-186 ,406-418
Function
Function in SwissProt
Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.