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IID50141
 UniprotP25554
ProteinSAGA-associated factor 29
GeneSGF29
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c)
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
 Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
259
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 111-259 Monomer : IID90020Complex
 Evidence X-RAY 3mp8 A Reference
       Region 3mp8 A 111-250 order
       Region 3mp8 A 251-259 disorder
Seq 111-259 Hetero dimer : IID00062Complex,IID90020Complex
 Evidence X-RAY 3mp1 A Reference
       Region 3mp1 A 111-137 order
       Region 3mp1 A 138-141 disorder
       Region 3mp1 A 142-232 order
       Region 3mp1 A 233-236 disorder
       Region 3mp1 A 237-254 order
       Region 3mp1 A 255-259 disorder
Seq 111-259 Hetero dimer : IID50143Complex,IID90020Complex
 Evidence X-RAY 3mp6 A Reference
       Region 3mp6 A 111-136 order
       Region 3mp6 A 137-141 disorder
       Region 3mp6 A 142-232 order
       Region 3mp6 A 233-236 disorder
       Region 3mp6 A 237-254 order
       Region 3mp6 A 255-259 disorder
Seqphosphorylation
    139-139 Phosphoserine
 
Prediction
NeProc
Disorder 65-122,255-259
Order 1-64,123-136,141-168,174-254
ProS 65-78,119-122
AlphaFold
Disorder 1-2,28-31,77-108,137-141,170-170,172-172,190-191,256-259
Order 3-27,32-76,109-136,142-169,171-171,173-189,192-255
Pfam Hmmer
PF07039 7-251 0.00093
SEG 84-96
Function
Function in SwissProt
Chromatin reader component of the transcription regulatory histone acetylation (HAT) complexes SAGA and SLIK (PubMed:10026213, PubMed:15647753, PubMed:21685874, PubMed:24307402). In the SAGA complex, SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PubMed:21685874). SGF29 is also required for heterochromatin boundary formation function (PubMed:24307402). SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes At the promoters, SAGA is required for recruitment of the basal transcription machinery (PubMed:10026213). It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8) (PubMed:10026213). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs) (PubMed:10026213). SLIK is proposed to have partly overlapping functions with SAGA (PubMed:15647753). It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus (PubMed:15647753).