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IID50313
UniprotP32558
ProteinFACT complex subunit SPT16
GeneSPT16
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c)
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
Network xml rdf
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
1035
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 1-465 Monomer :
 Evidence X-RAY 3bip A Reference
       Region 3bip A 1-3 disorder
       Region 3bip A 4-447 order
       Region 3bip A 448-465 disorder
 Evidence X-RAY 3bip B Reference
       Region 3bip B 1-4 disorder
       Region 3bip B 5-93 order
       Region 3bip B 94-95 disorder
       Region 3bip B 96-448 order
       Region 3bip B 449-465 disorder
 Evidence X-RAY 3biq A Reference
       Region 3biq A 1-4 disorder
       Region 3biq A 5-93 order
       Region 3biq A 94-95 disorder
       Region 3biq A 96-447 order
       Region 3biq A 448-465 disorder
 Evidence X-RAY 3bit A Reference
       Region 3bit A 1-447 order
       Region 3bit A 448-451 disorder
 Evidence X-RAY 3bit B Reference
       Region 3bit B 1-5 disorder
       Region 3bit B 6-94 order
       Region 3bit B 95-96 disorder
       Region 3bit B 97-106 order
       Region 3bit B 107-108 disorder
       Region 3bit B 109-447 order
       Region 3bit B 448-451 disorder
Seq 675-773,781-958 Monomer :
 Evidence X-RAY 4ioy X Reference
       Region 4ioy X 675-676 disorder
       Region 4ioy X 677-765 order
       Region 4ioy X 766-773 disorder
       Region 4ioy X 781-787 disorder
       Region 4ioy X 788-941 order
       Region 4ioy X 942-958 disorder
Seq 958-972 Hetero nonamer : IID50196Complex
 Evidence X-RAY 4wnn T Reference
       Region 4wnn T 958-966 disorder
       Region 4wnn T 967-972 order
Seqdisorder 973-990
 Evidence X-RAY Reference
       Region 973-990 disorder
SeqProS predicted 967-972 In the free status, the region 955-1035 is predicted to be disordered by multiple prediction tools (DICHOT, Mobi, FoldIndex, PrDOS) Hetero nonamer : IID50196Complex
       Region 4wnn T 967-972 order
Seqphosphorylation
    526-526 Phosphoserine
    765-765 Phosphoserine
 
Prediction
NeProc
Disorder 448-529,770-797,953-1035
Order 1-447,530-769,798-952
ProS 475-483,491-529,770-797,1014-1035
AlphaFold
Disorder 1-2,448-491,517-531,600-611,616-616,659-663,674-676,766-789,954-1017,1031-1035
Order 3-447,492-516,532-599,612-615,617-658,664-673,677-765,790-953,1018-1030
Pfam Hmmer
PF08644 553-707 3.1e-93
PF08512 851-1001 1.8e-16
SEG 448-458 ,773-797 ,957-1005 ,1009-1021
Function
Function in SwissProt
Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. Transcription elongation is promoted by the repression of transcription initiation from cryptic sites. Also acts in establishing transcription initiation complexes and promotes SPT15/TBP-binding to a TATA box. Together with replication factor-A protein (RPA), FACT may play a role in nucleosome deposition during DNA replication.
Biological Process
Diagram with PDB data
SPT16/HTB1/HTA1SPT16-H2A-H2B FACT HISTONE Complex