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IID50326
UniprotQ04636
ProteinFACT complex subunit POB3
GenePOB3
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c)
Sequence LLPS PhaSepDB
PhaSePro
LLPSDB
DrLLPS
xml
Structure
Experiment
  :order   disorder   conflict   PDB cluster   ProS   Pfam Domain   SEG
552
 order/disorder by at least rule
     disorder by at least rule
     order by at least rule
 order/disorder by majority rule
Seq 1-168 Monomer :
 Evidence X-RAY 3f5r A Reference
       Region 3f5r A 1-111 order
       Region 3f5r A 112-168 disorder
Seq 220-478 Monomer :
 Evidence X-RAY 4pq0 A Reference
       Region 4pq0 A 232-423 order
       Region 4pq0 A 424-432 disorder
       Region 4pq0 A 433-473 order
 Evidence X-RAY 2gcl B Reference
       Region 2gcl B 220-237 disorder
       Region 2gcl B 238-425 order
       Region 2gcl B 426-432 disorder
       Region 2gcl B 433-475 order
       Region 2gcl B 476-478 disorder
 Evidence X-RAY 2gcl A Reference
       Region 2gcl A 220-236 disorder
       Region 2gcl A 237-424 order
       Region 2gcl A 425-432 disorder
       Region 2gcl A 433-474 order
       Region 2gcl A 475-478 disorder
 Evidence X-RAY 2gcj D Reference
       Region 2gcj D 220-237 disorder
       Region 2gcj D 238-424 order
       Region 2gcj D 425-432 disorder
       Region 2gcj D 433-474 order
       Region 2gcj D 475-478 disorder
 Evidence X-RAY 2gcj C Reference
       Region 2gcj C 220-235 disorder
       Region 2gcj C 236-424 order
       Region 2gcj C 425-432 disorder
       Region 2gcj C 433-474 order
       Region 2gcj C 475-478 disorder
 Evidence X-RAY 2gcj B Reference
       Region 2gcj B 220-236 disorder
       Region 2gcj B 237-424 order
       Region 2gcj B 425-432 disorder
       Region 2gcj B 433-474 order
       Region 2gcj B 475-478 disorder
 Evidence X-RAY 2gcj A Reference
       Region 2gcj A 220-237 disorder
       Region 2gcj A 238-424 order
       Region 2gcj A 425-432 disorder
       Region 2gcj A 433-474 order
       Region 2gcj A 475-478 disorder
 
Prediction
NeProc
Disorder 182-218,478-552
Order 1-36,40-159,165-181,219-477
ProS 182-185,478-487,494-500
AlphaFold
Disorder 33-42,181-217,233-239,425-431,487-552
Order 1-32,43-180,218-232,240-424,432-486
Pfam Hmmer
PF03531 252-468 1.3e-139
SEG 330-347
Function
Function in SwissProt
Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. Transcription elongation is promoted by the repression of transcription initiation from cryptic sites. Also acts in establishing transcription initiation complexes and promotes SPT15/TBP-binding to a TATA box. Together with replication factor-A protein (RPA), FACT may play a role in nucleosome deposition during DNA replication.
Biological Process
See also
Diagram with PDB data
SPT16/HTB1/HTA1SPT16-H2A-H2B FACT HISTONE Complex